Download e-book for kindle: Advances in Microbial Ecology by Norman R. Pace, David A. Stahl, David J. Lane (auth.), K. C.

By Norman R. Pace, David A. Stahl, David J. Lane (auth.), K. C. Marshall (eds.)

ISBN-10: 1475706111

ISBN-13: 9781475706116

ISBN-10: 1475706138

ISBN-13: 9781475706130

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The evaluation oflabel incorporation into oligonucleotides unique to a particular 5S rRNA sequence scores its abundance in the original mixed population and provides an estimate of the abundance of the organisms that contributed that rRNA. This can only be an estimate, however, since rRNA content per cell is known to vary with metabolic status and between different cell types. 5. 1. Overview The relationships of the procaryotic 5S rRNAs recovered from Octopus Spring, the marine invertebrates, and the Chino Mine pond are summarized in the phylogenetic trees shown in Fig.

5S rRNA-derived relationships among the purple photosynthetic bacteria. The natural population constituents so far characterized by 5S rRNA analysis (bold lettering) mostly fall into the "purple photosynthe tic" group of the eubacteria [see inset (adapted from Stackebrand t and Woese, 1981) and text]. The sequences were aligned and the tree inferred as described by Stahl eta!. (1984) (also see text). The 5S rRNA sequences are from Erdmann eta!. (1984), Stahl eta!. (1984), Lane eta!. (1985), and N.

Hybridization conditions of sufficiently low stringency (conditions that stabilize hybrids that contain some mispaired bases) can detect 16S rRNA sequences over broad phylogenetic distances. Minimally, it is essential that the rRNA representative of one kingdom cross-hybridize with all other representatives of that kingdom. Since we cannot exhaustively inspect all representatives of a specific kingdom, this criterion is best demonstrated by the the ability to detect hybridization to homologous sequences across kingdom-level divisions.

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Advances in Microbial Ecology by Norman R. Pace, David A. Stahl, David J. Lane (auth.), K. C. Marshall (eds.)

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