Krishnarao Appasani, Sir E.M. Southern's Bioarrays: From Basics to Diagnostics PDF

By Krishnarao Appasani, Sir E.M. Southern

ISBN-10: 1588294765

ISBN-13: 9781588294760

This booklet offers an built-in selection of well timed articles at the use of bioarray thoughts within the fields of biotechnology and molecular drugs. it's the first booklet to comprehensively combine molecular diagnostics and molecular pathology. This ebook serves as an integral reference for graduate scholars, post-docs, and professors in addition to an explanatory research for executives and scientists in biotechnology and pharmaceutical businesses.

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Natl. Acad. Sci. USA 93, 10,614–10,619. 6. Lockhart, D. , Byrne, M. , et al. (1996) Expression monitoring by hybridization to high-density oligonucleotide arrays. Nat. Biotechnol. 14, 1675–1680. 7. Lipshutz, R. , Fodor, S. , Gingeras, T. , and Lockhart, D. J. (1999) High density synthetic oligonucleotide arrays. Nat. Genet. 21, 20–24. 8. , et al. (1998) Gene expression profiling of alveolar rhabdomyosarcoma with cDNA microarrays. Cancer Res. 58, 5009–5013. 9. , Wright, F. , Tanner, S. , et al. (2001) Expression profiling reveals fundamental biological differences in acute myeloid leukemia with isolated trisomy 8 and normal cytogenetics.

36. , Dougherty, E. , and Bittner, M. L. (1997) Ratio-based decisions and the quantitative analysis of cDNA microarray images. J Biomed. Opt. 2, 364–374. 37. Butte, A. (2002) The use and analysis of microarray data. Nat. Rev. Drug Discov. 1, 951–960. 38. Yang, I. , Hasseman, J. , et al. (2002) Within the fold: assessing differential expression measures and reproducibility in microarray assays. Genome Biol. 13. 39. , and Churchill, G. A. (2003) Statistical tests for differential expression in cDNA microarray experiments.

In contrast, microarray technology (13) has the capacity for assessing multiple samples, thereby generating specific gene expression profiles characteristic of a given tumor class. In short, this technology consists of an ordered collection of DNA fragments (cDNA or oligonucleotides) immobilized on a solid surface. The immobilized sequences represent genes, and each microspot is composed of a single species of sequences. The technology is based on the hybridization between DNA sequences immobilized on the slide and labeled free sequences generated from the mRNA population from a given biological sample.

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Bioarrays: From Basics to Diagnostics by Krishnarao Appasani, Sir E.M. Southern

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