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In addition to influencing the spot morphology, the spotting solution should stabilize printing solution system, lead to consistent and stable spots on the surface, and allow for a high efficiency of the immobilization. Procedures on how to optimize a solvent for spotting cDNA are described (1,2). However, as surface properties strongly depend on the specific experimental conditions during the surface-coating process, substrates that in principle have the same surface architecture but are obtained from a variety of manufacturers can feature different slide properties.

Koehler, A. , and Schreiber, S. L. (1999) Printing small mole52. cules as microarrays and detecting protein-ligand interactions en masse. J. Am. Chem. Soc. 121, 7967–7968. 53 Hergenrother, P. , Depew, K. , Schreiber, S. L. (2000) Small-molecule 53. microarrays: covalent attachment and screening of alcohol-containing small molecules on glass slides. J. Am. Chem. Soc. 122, 7849–7850. 54 Koehler, A. , Shamji, A. , and Schreiber, S. L. (2003) Discovery of an 54. inhibitor of a transcription factor using small molecule microarrays and diversityoriented synthesis.

8. Anti-fluorescein antibody prepared by Dr. S. Melkko (ETH Zurich). 9. Model peptide 5(6)-Carboxyfluorescein-KTKESLGRKIQIQRSG-NH2, gift from Dr. M. Schutkowski (JPT Peptide Technologies, Berlin). 10. Sodium chloride (molecular biology grade) from Sigma-Aldrich (Buchs, CH). 11. 5%) from Sigma-Aldrich (Buchs, CH). 12. 0%) from Sigma-Aldrich (Buchs, CH). 13. Trisodium citrate (>99%) from Sigma-Aldrich (Buchs, CH). 14. Fluorescein isothiocyanate dextrane conjugate (molar mass 4000 g/mol) from Fluka, (Buchs, CH).

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Data Embedding and Digital Watermarking by Su

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